EGFP protein

Storage

For short term storage EGFP protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
Tips

Due to handling and transportation, small amounts of the EGFP protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
Conjugate

The Green Fluorescent Protein (eGFP) of protein can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.

Identity

HGNC:10311
Gene

RPL18A
Long gene name

ribosomal protein L18a
Synonyms
- L18A
Synonyms name
- 60S ribosomal protein L18a
- ribosomal protein L18a-like protein
GenBank acession
- AB007175
Locus

19p13.11
Discovery year

1998-08-18
Entrez gene record

6142
Pubmed identfication
- 9582194
RefSeq identity
- NM_000980
Classification
- Small nucleolar RNA protein coding host genes
- L ribosomal proteins
VEGA ID

OTTHUMG00000183445