Blue Fluorescent Protein (BFP)
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Catalog number
4994-5000
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Price
Please ask
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Size
5 mg
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Synonyms
BFP, Blue Fluorescent Protein
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Alternative_names
BFP, Blue Fluorescent Protein
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Description
Fluorescent protein ideal for subcellular labeling/visualization
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Purity
≥97%
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Molecular Weight
29.0 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Freeze Dried
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Reconstitution Instructions
Reconstitute with dH₂O to 1 mg/ml
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Background Information
The recombinant Blue Fluorescent Protein (BFP) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal BFP fluorescence. The protein is a 29 kDa monomer with 259 amino acids, pI: 6.17. Ex.= 308-383 nm; Em.= 440-447 nm. The protein is engineered with 6xHis-tag on the N-terminal, which can be used for detection with anti-His-Tag antibody, or protein removal by using Ni++ beads. BFP protein sequence:MGSSHHHHHHSSGLVPRGSHMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY GKLTLKFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
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Conjugation
Blue Substrate
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Additional description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
Gene info
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Identity
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Gene
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Long gene name
ring finger protein 112
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Synonyms gene
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1995-10-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Product images
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