PFU DNA Polymerase

Synonyms

DNA polymerease
Alternative_names

DNA polymerase (EC 2.7.7.7) (Pfu polymerase), pol PF0212
Description

An enzyme that catalyzes the polymerization of nucleotides
Recombinant

Yes
Source

E. coli
Assay

SDS-PAGE
Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.
Storage Temp

-20°C
Shipping

Gel Pack
Shelf Life

12 months
Concentration

2.5 U/µl
Appearance

Liquid
Physical form description

ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Tween 20, and 0.5% Nonidet P40.
Background Information

BioVision’s Pfu DNA Polymerase, combined with optimized buffer for high-fidelity and yield, provides the most accurate and consistent performance in PCR. BioVision’s enzyme demonstrates up to 20 folds greater accuracy than other similar enzyme in the market. BioVision’s Pfu DNA Polymerase can be used directly in any protocol requiring Pfu DNA Polymerase. The enzyme concentration and reaction buffer are already optimized for most published high-fidelity PCR protocols.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet