NuCycl DAPI
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Catalog number7034
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PricePlease ask
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Size50 Tests
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CategoryDNA Staining Kit
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Long descriptionNuCycl DAPI (4', 6 - diamidino -2 phenylindole - 2 HCL) - DNA specific stain procedure. NuCycl DAPI allows the rapid isolation of nuclei from cells and tissues. NuCycl DAPI stains only DNA and DOES NOT stain RNA.
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Antibody come fromn/a
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Other descriptionNo other information available
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Clonen/a
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Antigen antibody binding interactionNuCycl DAPI DNA Staining Kit
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Antibody is raised inn/a
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Antibody s reacts withn/a
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Antibody s reacts with these speciesn/a
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Antibody s specificityn/a
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Research interestDNA staining
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ApplicationFlow Cytometry
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Antibody s suited forAssay Procedure: The following procedure is recommended for the preparation of cells or tissues for DNA Flow Cytometry. Set instrument or analyzer for DNA/RNA analysis. See instrument manufacturers instructions for proper DNA/RNA procedure. Note: Exalpha recommends that the NuCycl Sample Preparation filters be used for the preparation of all samples and standards to be run. NuCycl Sample Preparation Filters remove background noise and allow for tighter CV's and hence better sensitivity than would otherwise be possible.
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Storage4ÂșC
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Relevant references1. Thornthwaite, J.T., Thomas, R.A. High - Resolution DNA Measurements Using the Nuclear Isolation Medium, DAPI, with the RATCOM Flow Cytometer. Methods in Cell Biology 33:111-119, 1990. _x000B__x000B_2. Hornicek, F.J., Thornthwaite, J.T., Malinin, G.I., Seckinger, D., and Malinin, T.I. Evaluation of 4,6 - diamidino-2-phenylindole (DAPI) for Flow Cytometry of Blastogenesis. _x000B__x000B_3. Thornthwaite, J.T., et al. A Review of DNA Flow Cytometric Preparatory and Analytical Methods. Immunocytochemistry in Tumor Diagnosis. Ed. J. Russo. Martinus Nijhoff: Boston pp. 380-398, 1985. _x000B__x000B_4. Lee, G.M., Thornthwaite, J.T., and Rasch, E.M. Cytometric Comparisons of DNA Levels in Neuronal and Glial Cells of the Cerebellum. Cell Biochem. Function. 33:262-270, 1985. _x000B__x000B_5. Lee, G.M., Thornthwaite, J.T., and Rasch, E.M. Picogram Per Cell Determination of DNA by Flow Cytofluorometry. Anal. Biochem. 137:221-226, 1984. _x000B__x000B_6. Coulson, P.B., Thornthwaite, J.T., Wooley, T., Seckinger, D., and Sugarbaker, E.V. Prognostic Indicators Including DNA Histogram Type Receptor Content, and Staging Related to Human Breast Cancer Patient Survival. Cancer Research. 44:4187-4196, 1984. _x000B__x000B_7. Youngberg, G.A., Thornthwaite, J.T., and Fanzus, D. Cytologically Malignant Squamous-Cell Carcinoma of the Penis. J. Dermtol. Surg. Oncol. 9:474-479, 1983. _x000B__x000B_8. Evans, D., Thornthwaite, J.T., Ng A., and Sugarbaker, E.V. DNA Flow Cytometry of Human Pleural Effusions: Comparison with pathology for the Diagnosis of Malignancy. Anal. Quant. Cyto. 5:19-27, 1983. _x000B__x000B_9. Thornthwaite, J.T., Sugarbaker, E.V., Temple W.J. Preparations of Tissues for DNA Flow Cytometric Analysis. Cytometry. 1:229-237, 1980. _x000B__x000B_10. Allen, L.M., Thornthwaite, J.T. Studies on the Pharmacology and Cytokinetics of Imidazole Pyrazole (NSC-51143) with p815 Mastocytoma Cells. Cancer Research 40:4059-1063, 1980. _x000B__x000B_11. Thornthwaite, J.T., Allen, L.M. The Effect of Glutamine Analog, AT-125, on the Cell Cycle of MCF-7 and BT-20 Human Breast Carcinoma Cells Using DNA Flow Cytometry. Res. Comm. Chem. Path. 29:393-396, 1980. _x000B__x000B_12. Temple, W.J., Sugarbaker, E.V., Thornthwaite, J.T., Hensley, G.T., Ketcham, A.S. Correlation of Cell Cycle Analysis with Duke's Staging in Colon Cancer Patient. J. Surg. Res. 28:314-318, 1980. _x000B__x000B_13. Sugarbaker, E.V., Thornthwaite, J.T., Temple, W.J., Ketcham, A.S. Flow Cytometry: General Principals and Applications to Selected Studies in Tumor Biology. Int. Adv. Surg. Oncol. 2:125-153.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Gene target
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Short nameNuCycl DAPI
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Isotypen/a
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Labelunlabeled
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Alternative nameNuCycl DAPI
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