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Melanoma Lysate

  • Catalog number
    20-101
  • Product name
    Melanoma Lysate
  • Size
    0.1 mg
  • Price
    415EUR
  • Supplier
    proscience
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  • Lysates properties
    Melanoma lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 grades C. The human melanoma tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the melanoma tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The melanoma tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
  • Lysate's origin
    Tissue
  • State of tissue
    Diseased
  • Species of the lysate
    Human
  • Cellular origibn of the lysate
    Skin
  • Disease of the lysate
    Tumor
  • Localisation of the biopsy
    Human Skin tissue
  • Ug of lysate used by test
    Melanoma lysate is for use in Western blotting, 10 ug to 20 ug per lane is recommended for mini gel.
  • Positive control in
    the lysate is used as positive control in Western Blot
  • PH and medium
    HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
  • Keep at
    Store at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
  • Caution
    This product is for research use only.
  • Laboratory requirements
    Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
  • Animal origin
    Human
  • Dilution
    2 mg/mL or more concentrated
  • Gene target
  • Short name
    Melanoma Lysate
  • Alternative name
    Melanoma Lysate
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MESH Data
  • Name
  • Concept
    Scope note: Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.
  • Tree numbers
    • C04.588.531.500
    • C04.619.590
    • E05.598.500.496.843
  • Qualifiers
    parasitology, immunology, microbiology, diagnosis, diagnostic imaging, physiopathology, secondary, secretion, enzymology, etiology, mortality, epidemiology, prevention & control, blood, cerebrospinal fluid, chemically induced, congenital, diet therapy, drug therapy, therapy, chemistry, psychology, urine, virology, history, blood supply, classification, complications, economics, embryology, genetics, metabolism, nursing, radiotherapy, surgery, pathology, ultrastructure
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