Donkey, L), Biotin conjugated
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Catalog number
AS10 842
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Price
Please ask
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Size
2 mg
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Available ordering format
Liquid
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Immunogen
Purified Rabbit IgG
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Raised in
Donkey
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified donkey IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
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Verified applications
suitable for all immunoassays applications
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Connected products
AS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated
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Recommended dilutions for use
optimal working dilution should be determined by the investigator
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
rabbit IgG (H&L)
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Possible reactivity
rabbit IgG (H&L)
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
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References
to be added when available
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Scientific context
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin which binds to rabbit IgG (H&L) in immunological assays.
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Notes
biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Equus africanus asinus
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Gene target
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Short name
, L), conjugated
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Technique
antibody Conjugates
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Host
Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Label
Biotin
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Species
Donkeys
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Alternative name
Donkey, L), biotinilated coupled
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