Donkey, L), Biotin conjugated

• Catalog number
  AS09 611
• Price
  Please ask
• Size
  2 mg

• Available ordering format
  Liquid
• Immunogen
  Purified sheep IgG, whole molecule
• Raised in
  Donkey
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified donkey IgG
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
• Verified applications
  ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
• Connected products
  AS09 604 | Donkey anti-sheep IgG (H&L) HRP conjugated
• Recommended dilutions for use
  1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  sheep IgG heavy and light chains (H&L)
• Possible reactivity
  sheep IgG heavy and light chains (H&L)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
• References
  to be added when available
• Scientific context
  Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.
• Notes
  Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Latin name
  Equus africanus asinus

• Gene target
  conjugated  
• Short name
  , L), conjugated
• Technique
  antibody Conjugates
• Host
  Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Label
  Biotin
• Species
  Donkeys
• Alternative name
  Donkey, L), biotinilated coupled

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