Iscove's Modified DMEM (IMDM) w/ HEPES buffer w/o L-glutamine, phenol red, and sodium bicarbonate
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Catalog number
IMP05-50LT
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Price
Please ask
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Size
50 L
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Availability
Please contact us to check current availability
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Long description
Iscove's Modified DMEM (IMDM) w/ HEPES buffer w/o L-glutamine, phenol red, and sodium bicarbonate Medium
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Product format
Powder
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Product category
Animal Cell Culture Media
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Product subcategory
Iscove's Modified DMEM
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Shipping method
Ambient temperature
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Storage method
Store at 2˚ to 8˚C.
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Description
Buffering solutions are useful to keep the pH range sable when using this reagent of caissonlab.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Conjugation
red dye
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