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Available ordering format
Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
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Immunogen
BSA-conjugated synthetic indole 3 acetic acid
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 50 µl of sterile water and 50 µl of glycerol
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Storage condition
store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), immunolocalization (IL)
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Connected products
AS09 445 | anti-indole- 3- acetic acid (C1') for immunolocalizationAS09 420 | anti-indole 3 acetic acid (C1') (for ELISA)AS09 421 | anti-indole 3 acetic acid (N1)AS12 1865 | Ethylene insensitive 2collection of antibodies to plant hormones
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Recommended dilutions for use
1: 5000 - 1: 10 000 (ELISA), 1:100 - 1:600 (IL)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago sativa (nodules)
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Possible reactivity
dicots and monocots
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
For detailed immunolocalization protocol check here.Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).Whole-mount immunolocalization is described in Lu et al. (2015).
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References
Lu et al. (2015). OsPIN5b modulates rice plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015 Jul 25. doi: 10.1111/tpj.12939. Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.
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Scientific context
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
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Notes
total IgG was purified by ammonium sulfate precipiation
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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