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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide 6xHis
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG2b, clone HIS.H8 / EH158
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For storage at 4°C for several days to weeks, sodium azide to final 0.1 % can be added. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipitation (IP), immunlocalization (IL)
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Connected products
collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
6xHis
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Possible reactivity
6xHis
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
antibody is present in 10 mM PBS, pH 7.2
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References
to be added when available, antibody available since July 2011.
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Scientific context
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
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Notes
working dilution for ELISA, IL and IP needs to be determined experimentally.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Conjugated
The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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Conjugation
histidine
