Human EGFR Gene Mutations Detection Kit (Multiplex Fluorescence PCR)
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Catalog numberSpaceGen-005
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PricePlease ask
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Size1 Kit
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DescriptionHuman EGFR Gene Mutations Detection Kit (Multiplex Fluorescence PCR) is used to detect the EGFR mutation in a patient in ordre to determine if a patient can apply EGFR-TKI therapy, EGFR mutation is responsable for cancer cells proliferation and relevant signal transduction, erlotinib, icotinib and other EGFR-TKI and anti-EGFR antibodies have been developped for cancer treatment.
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Specifications10tests/kit
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Other NameDetection Kit for Human EGFR Gene Mutation
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Detection ProcessMultiplex Fluorescence PCR
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Samples TypesParaffin-embedded tissue, fresh tumor tissue, fresh frozen tumors, blood, pleural effusion, etc.
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GeneEGFR, EGFR-TKI
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ReactivityHuman
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Advantages and CharacteristicsEasy operation: this kit only takes one step to add the sample, which can avoid pollution and save time, and it takes only 90 minutes to complete the detectionComprehensive Inspection: This kit can effectively detect the common 29 kinds of mutational sites, Strong Specificity: This kit are able to tolerate DNA in the background of 100ng wild-type genome, without nonspecific amplification, High snsitivity: This kit can accurately detect DNA samples with a mutation content of 0.1% in the background of 10ng genome.
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UseCE-IVD
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Additional descriptionThe detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
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TestFluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Gene target
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Gene symbolEGFR-AS1, EGILA, EGFR, RHBDF1
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Short nameEGFR Mutations Kit (Multiplex PCR)
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Techniquedetection, multiplex, PCR, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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SpeciesHuman, Humans
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Alternative nameGene Mutations Detection Kit
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Alternative techniquepcrkits, kits, dna-amplification
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Alternative to gene targetepidermal growth factor receptor, ERBB and ERBB1 and HER1 and mENA and PIG61, EGFR and IDBG-16933 and ENSG00000146648 and 1956, transferase activity, nuclei, Egfr and IDBG-151194 and ENSMUSG00000020122 and 13649, EGFR and IDBG-644035 and ENSBTAG00000011628 and 407217
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DiseaseLung Cancer, varietyof cancer
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Gene info
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Identity
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Gene
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Long gene nameEGFR antisense RNA 1
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Locus
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Discovery year2012-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEGFR interacting lncRNA
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Synonyms
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Locus
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Discovery year2019-09-27
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Pubmed identfication
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Classification
- Long non-coding RNAs with non-systematic symbols
Gene info
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Identity
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Gene
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Long gene nameepidermal growth factor receptor
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Synonyms gene
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Synonyms gene name
- epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Erb-b2 receptor tyrosine kinases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namerhomboid 5 homolog 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 8
- rhomboid family 1 (Drosophila)
- rhomboid 5 homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Rhomboid family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
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Tree numbers
- E05.393.620.500.487
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data