AO, ascorbate oxidase

  • Catalog number
    AS09 384
  • Price
    Please ask
  • Size
    1 mg
  • Available ordering format
    Lyophilized in PBS pH 7.2
  • Immunogen
    ascorbate oxidase purified from Cucurbita sp.
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Total IgG
  • How to reconstitute
    For reconstitution add 0.5 ml of sterile water
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Verified applications
    Western blot (WB), ELISA (ELISA), immunohistochemistry (IHC)
  • Connected products
    AS06 184 | anti-ascorbate oxidase rabbit antibodyAS09 383 | anti-ascrobate oxidase biotinylated rabbit antibodyPlant and algal protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 1000 (WB), 1: 1000 - 1: 30 000 (ELISA)
  • Molecular weight expected аpparent
    61 | 45 kDa (Cucurbita pepo)
  • Verified reactivity
    Cucurbita sp.
  • Possible reactivity
    dicots including Glycine max, monocots including Oryza sativa
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    to be added when available
  • References
    to be added when available
  • Scientific context
    Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
  • Notes
    IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Gene target
  • Short name
    AO, ascorbate oxidase
  • Alternative name
    AO, ascorbate oxidase
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