5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)
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Catalog number
GEN644609
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Price
Please ask
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Size
100ug
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Also known as
5-Hydroxymethylcytosine
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Other names
5-hydroxymethylcytosine (hmC) binding, ES cell-specific; N/A; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
hmces; N/A; im:7137789; zgc:165500; N/A
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Gene name synonims
hmces; N/A; im:7137789; zgc:165500; N/A
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Other gene names
hmces; N/A; im:7137789; zgc:165500; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a,k
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Clone
AB3/63.3
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Host organism
Rat (Rattus norvegicus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested applications
Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
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Description
A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
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Gene target
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Short name
5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)
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Alternative name
Anti-5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)
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