3-D Culture Laminin I

Keep at

-20° C
Reagent list
Assay performance

Cultrexes are the best 3D matrixes for cell cultures known as matrigel or cultrex. Basal membrane extracts higher the differentiation capacity or from basal membrane over what can be achieved by 2D monolayer cultures from epithelial cells. You can use cultrex for high differentiation in a 3D matrix as Basement Membrane Extracts (BME), extracellular matrix from lumen epithelial or endothelial cells. BME, collagen I, collagen IV, laminin I, fibronectin and vitronectin are pathogen tested and suitable for 3d and pluripotent stem cell culture. Cultrex Basement Membrane and the PathClear Protocol is supplied in a liquid form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor at a concentration of 13 - 17 mg/ml from Murine Engelbreth-Holm-Swarm (EHS) tumor with Dulbecco’s Modified Eagle’s medium without phenol red and with 11 ug/ml gentamicin sulfate with cultrex BME for thin Layer Method (non-gelling).
More advice

Cultrex 3D cell culture for cancer studies cell invasion and 3 dimensional cell expansion up to 2/3 conflunecy
Other trevi products

Identity

HGNC:17226
Gene

DGCR11
Long gene name

DiGeorge syndrome critical region gene 11
Synonyms gene name
- DiGeorge syndrome critical region gene 11
- DiGeorge syndrome critical region gene 11 (non-protein coding)
Synonyms
- DGS-D
GenBank acession
- L77561
Locus

22q11.21
Discovery year

2002-11-13
Entrez gene record

25786
Pubmed identfication
- 8776594
Classification
- Long non-coding RNAs with non-systematic symbols
VEGA ID

OTTHUMG00000186100

Identity

HGNC:13935
Gene

LY6G6D
Long gene name

lymphocyte antigen 6 family member G6D
Synonyms gene
- C6orf23
Synonyms gene name
- chromosome 6 open reading frame 23
- lymphocyte antigen 6 complex, locus G6D
Synonyms
- MEGT1
- Ly6-D
- G6D
- LY6-D
- NG25
Locus

6p21.33
Discovery year

2002-04-05
Entrez gene record

58530
Pubmed identfication
- 12079290
Classification
- LY6/PLAUR domain containing
VEGA ID

OTTHUMG00000137370

Identity

HGNC:15776
Gene

ULK4P2
Long gene name

ULK4 pseudogene 2
Synonyms gene
- FAM7A2
Synonyms gene name
- family with sequence similarity 7, member A2
- unc-51-like kinase 4 (C. elegans) pseudogene 2
Synonyms
- D-X
Locus

15q13.2
Discovery year

2001-06-29
Entrez gene record

100288380
Pubmed identfication
- 11829490
VEGA ID

OTTHUMG00000175653

Identity

HGNC:15775
Gene

ULK4P1
Long gene name

ULK4 pseudogene 1
Synonyms gene
- FAM7A1
Synonyms gene name
- family with sequence similarity 7, member A1
- family with sequence similarity 7, member A1 (non-protein coding)
- unc-51-like kinase 4 (C. elegans) pseudogene 1
Synonyms
- D-X
GenBank acession
- BC070492
Locus

15q13.3
Discovery year

2001-06-29
Entrez gene record

89838
Pubmed identfication
- 11829490
RefSeq identity
- XR_041690
VEGA ID

OTTHUMG00000162940

Identity

HGNC:3708
Gene

VEGFD
Long gene name

vascular endothelial growth factor D
Synonyms gene
- FIGF
Synonyms gene name
- c-fos induced growth factor (vascular endothelial growth factor D)
Synonyms
- VEGF-D
GenBank acession
- AJ000185
Locus

Xp22.2
Discovery year

1997-03-27
Entrez gene record

2277
Pubmed identfication
- 9479493
RefSeq identity
- NM_004469
Classification
- VEGF family
VEGA ID

OTTHUMG00000021175
Locus Specific Databases
- Global Variome shared LOVD

Name

Culture Techniques
Concept

Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Tree numbers
- E05.481.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data