Bead Sample Pack - Cell Culture used

Bulletblenders sonication use

Consumable
The use of this ultrasonic sonicator

Bullet Blender
Weight of the sonicator or reagent

0.5
Harmonized code of the instrument

7326.11.0000
Nature of the sonication instrument or reagent for cell lyzis

Bead sample pack for homogenization of cell cultures. Includes 10mL of: GB01, GB05, ZROB015, ZROB05 and SSB02.
Unit of homogenizing sonnicator sold

pack
Technical cell or tissue disruption

Homogenisation and disruption can be achieved by the mechanical Bullet blenders in a simular way but with less heating as with sonicators for cellular disruption and lysis. Instread of using sonication with utrasonic disruption of cells or tissues, the bullet blender technique and bullet blender gold will use gentle and heat absent cell lysis. Bullet Blenders can use more than 50gr of cell quantities. Homogenisers of French press for e.Coli lysation are less performant than bullet blenders even with 2 passages at 15,000 psi! DNAse can be added to preserve DNA for degradation for Epigenetics applications or for PCR.
Technical information about the Bullet Blenders
Description

For cells, cell lines and tissues in culture till half confluency.

Name

Culture Techniques
Concept

Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Tree numbers
- E05.481.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data