Recombinant Human Junctional Adhesion Molecule A/JAM-A/CD321 (C-6His)

• Catalog number
  C468-50
• Price
  Please ask
• Size
  50 ug

• Description
  Recombinant Human JAM-A is produced by our Mammalian expression system and the target gene encoding Ser28-Val238 is expressed with a 6His tag at the C-terminus.
• Species reactivity
  Human
• Origin
  Human cells
• Peptide sequence
  SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVDHHHHHH
• Estimated molecular weight
  23,89 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Ambient/Room Temperature
• Package form
  Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl, 50mM NaCl, 100mM Glycine, pH 7.5.
• Storage conditions
  Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  Q9Y624
• Other related products
• Additional description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Recombinant Junctional Adhesion Molecule A/JAM-A/CD321 (C-6His). Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Junctional   Adhesion   Molecule   A/JAM-A/CD321   C-6His  
• Gene symbol
  F11R, JAM2, JAM3, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1
• Short name
  Recombinant Junctional Adhesion Molecule A/JAM-A/CD321 (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human JAM-A/F11 Receptor/CD321(C-6His)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:14685
• Gene
  F11R
• Long gene name
  F11 receptor
• Synonyms gene
  • JAM1
• Synonyms gene name
  • junctional adhesion molecule 1
• Synonyms
  • PAM-1
  • JCAM
  • JAM-1
  • JAM-A
  • JAMA
  • CD321
• GenBank acession
  • AF111713
• Locus
  1q23.3
• Discovery year
  2001-04-10
• Entrez gene record
  50848
• Pubmed identfication
  • 10395639
  • 7646439
• RefSeq identity
  • NM_016946
• Classification
  • Ig-like cell adhesion molecule family
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000028602

Gene info

• Identity
  HGNC:14686
• Gene
  JAM2
• Long gene name
  junctional adhesion molecule 2
• Synonyms gene
  • C21orf43
• Synonyms
  • VE-JAM
  • JAM-B
  • JAMB
  • CD322
• GenBank acession
  • AF255910
• Locus
  21q21.3
• Discovery year
  2001-04-25
• Entrez gene record
  58494
• Pubmed identfication
  • 10779521
  • 10945976
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • I-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000078441

Gene info

• Identity
  HGNC:15532
• Gene
  JAM3
• Long gene name
  junctional adhesion molecule 3
• Synonyms
  • JAM-C
  • JAMC
• GenBank acession
  • AF356518
• Locus
  11q25
• Discovery year
  2001-04-26
• Entrez gene record
  83700
• RefSeq identity
  • NM_032801
• Classification
  • Small nucleolar RNA protein coding host genes
  • Ig-like cell adhesion molecule family
  • V-set domain containing
• VEGA ID
  OTTHUMG00000167130

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

Gene info

• Identity
  HGNC:1976
• Gene
  CIDEA
• Long gene name
  cell death inducing DFFA like effector a
• Synonyms gene name
  • cell death-inducing DFFA-like effector a
• Synonyms
  • CIDE-A
• GenBank acession
  • AF041378
• Locus
  18p11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1149
• Pubmed identfication
  • 9564035
  • 18509062
• RefSeq identity
  • NM_001279
• VEGA ID
  OTTHUMG00000131691

Gene info

• Identity
  HGNC:10289
• Gene
  RPA1
• Long gene name
  replication protein A1
• Synonyms gene name
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
• Synonyms
  • REPA1
  • RPA70
  • HSSB
  • RF-A
  • RP-A
• GenBank acession
  • M63488
• Locus
  17p13.3
• Discovery year
  1993-12-14
• Entrez gene record
  6117
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002945
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000090579

Gene info

• Identity
  HGNC:34406
• Gene
  CDC37P1
• Long gene name
  cell division cycle 37 pseudogene 1
• Synonyms gene name
  • cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
• Synonyms
  • A-761H5.2
• Locus
  16p11.2
• Discovery year
  2009-01-29
• Entrez gene record
  390688
• Pubmed identfication
  • 15616553
• RefSeq identity
  • NG_009463
• VEGA ID
  OTTHUMG00000175739

Gene info

• Identity
  HGNC:8223
• Gene
  OR1Q1
• Long gene name
  olfactory receptor family 1 subfamily Q member 1
• Synonyms gene
  • OR1Q2
  • OR1Q3
• Synonyms gene name
  • olfactory receptor, family 1, subfamily Q, member 1
• Synonyms
  • OST226
  • OR9-A
  • HSTPCR106
  • OST226OR9-A
  • TPCR106
• Locus
  9q33.2
• Discovery year
  1999-12-09
• Entrez gene record
  158131
• Classification
  • Olfactory receptors, family 1
• VEGA ID
  OTTHUMG00000020615

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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