Rabbit anti Sheep erythrocyte
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Catalog numberRASh/Ery/7S
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PricePlease ask
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Size10 mg
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CategoryPrimary Antibodies
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Long descriptionTo sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
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Antibody come fromWashed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionPurified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti Sheep erythrocyte Antibody
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Antibody is raised inRabbit
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Antibody s reacts withSheep
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Antibody s reacts with these speciesHomologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.
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Antibody s specificityIgG fraction of polyclonal Rabbit antiSerum to Sheep erythrocytes.
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Research interestVeterinary
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ApplicationIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting
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Antibody s suited forIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x108 RBC/ml, not less than 1:3,000 when tested with sheep erythrocytes.
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StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolEPB42, EPB41L4A, EPB41L4B
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Short nameRabbit anti erythrocyte
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TechniqueRabbit, anti, antibody to
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HostRabbit, Rabbits
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Isotypenot specified
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Labelunlabeled
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SpeciesSheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative nameproduction species: rabbit antibody to ovine erythrocyte
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Alternative techniquerabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameerythrocyte membrane protein band 4.2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transglutaminases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameerythrocyte membrane protein band 4.1 like 4A
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-11-27
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Entrez gene record
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Pubmed identfication
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Classification
- FERM domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameerythrocyte membrane protein band 4.1 like 4B
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- FERM domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data