Sheep anti Rabbit Glyceraldehyde-3-phosphate dehydrogenase
-
Catalog numberNE066/7S
-
PricePlease ask
-
Size10 mg
-
-
CategoryPrimary Antibodies
-
Long descriptionThe reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
-
Antibody come fromGlyceraldehyde-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
-
Other descriptionPurified hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
-
ClonePolyclonal
-
Antigen antibody binding interactionSheep anti Rabbit Glyceraldehyde-3-phosphate dehydrogenase Antibody
-
Antibody is raised inSheep
-
Antibody s reacts withRabbit
-
Antibody s reacts with these speciesCross-reactivities anti enzymes of other sources may occur but have not been determined.
-
Antibody s specificityIgG fraction of polyclonal Sheep antiSerum to glyceraldehyde-3-phosphate dehydrogenase from Rabbit muscle.
-
Research interestEnzymes, Veterinary
-
ApplicationIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting
-
Antibody s suited forIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/10 and 1/300.
-
StorageThe lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
-
Relevant referencesno information yet
-
Protein numberP46406
-
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Gene symbolMIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC12, PIRC14
-
Short nameanti Rabbit Glyceraldehyde-3-phosphate dehydrogenase
-
TechniqueRabbit, anti, antibody to
-
HostSheep, Rabbits
-
Isotypenot specified
-
Labelunlabeled
-
SpeciesSheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
-
Alternative nameovine antibody to production species: rabbit Glyceraldehyde-3-phosphate dehydrogenase
-
Alternative techniquerabbit-anti, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1302-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1302 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 509-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2007-10-24
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR506 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 7-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR7 family
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 13
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 11
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 12
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 14
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data