Rabbit anti Leptin (Rat)

• Catalog number
  X1834P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
• Antibody come from
  Baclovirus infected Sf9 cells
• Other description
  Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 3 mM DTT and 50% glycerol. Antigen Immunoaffiinity Purification
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Rabbit anti Leptin (Rat) Antibody
• Antibody is raised in
  Rabbit
• Antibody s reacts with
  Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  ELISA, Western Blot
• Antibody s suited for
  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity_x000B_profiling. Full lengtth mouse recombinant sphingosie Kinase 1a protein with N terminal His tag. 20U/ug specific activity A unit of activity determined as the amount ofenzyme required to produce 1 pmol of S1P/min. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine_x000B_and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.1-0.5 ug/ml sphingosine kinase 1.
• Storage
  -20ºC
• Relevant references
  1. Delon, C., etal., J. Biol. Chem 279 (43) 44763-44774 (2004)_x000B__x000B_2. Taha, TA etal., FASEB J. 20(3):482-4 (2005)_x000B_
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Gene
  Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Oryctolagus cuniculus, Rattus norvegicus

• Gene target
  Leptin  
• Gene symbol
  LEP, LEPR, LEPROT
• Short name
  Rabbit anti Leptin ( )
• Technique
  Rabbit, anti, antibody to
• Host
  Rabbit, Rabbits
• Isotype
  not specified
• Label
  unlabeled
• Species
  Rat, Rats
• Alternative name
  production species: rabbit antibody to Leptin (Rat)
• Alternative technique
  rabbit-anti, antibodies

Gene info

• Identity
  HGNC:6553
• Gene
  LEP
• Long gene name
  leptin
• Synonyms gene
  • OBS
  • OB
• Synonyms gene name
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
• Locus
  7q32.1
• Discovery year
  1993-01-26
• Entrez gene record
  3952
• Pubmed identfication
  • 1686014
  • 16932309
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000157564

Gene info

• Identity
  HGNC:6554
• Gene
  LEPR
• Long gene name
  leptin receptor
• Synonyms
  • OBR
  • CD295
• GenBank acession
  • U43168
• Locus
  1p31.3
• Discovery year
  1996-05-09
• Entrez gene record
  3953
• Pubmed identfication
  • 8548812
  • 8812446
• RefSeq identity
  • NM_002303
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000009115
• Locus Specific Databases
  • LRG_283

Gene info

• Identity
  HGNC:29477
• Gene
  LEPROT
• Long gene name
  leptin receptor overlapping transcript
• Synonyms
  • OBRGRP
  • OB-RGRP
  • VPS55
  • FLJ90360
• Synonyms name
  • leptin receptor gene related protein
  • endospanin-1
  • endospanin
• GenBank acession
  • Y12670
• Locus
  1p31.3
• Discovery year
  2004-11-16
• Entrez gene record
  54741
• Pubmed identfication
  • 9207021
  • 21454707
• RefSeq identity
  • NM_017526
• VEGA ID
  OTTHUMG00000009065

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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