Rabbit anti Mouse IgG2b FITC

  • Catalog number
    43R-IR013ft
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Rabbit anti-mouse IgG2b (FITC) was raised in rabbit using murine IgG2b heavy chain ..
  • Raised in
    Rabbit
  • Specificity
    Murine IgG2b
  • Cross Reactivity
    Should be determined by the end user
  • Conjugate Tag
    FITC
  • Method of Purification
    Rabbit anti Mouse IgG2b (FITC) was purified by affinity chromatography.
  • Form Buffer
    Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
  • Storage
    Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; FC; IF
  • Usage Recommendations
    ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Oryctolagus cuniculus
  • About
    Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Gene target
    IgG2b  
  • Short name
    Rabbit anti Mouse IgG2b FITC
  • Technique
    anti mouse, Rabbit, Mouse, anti, FITC, antibody to, anti mouse antibodies, Fluorescein, mouses
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG2b
  • Label
    FITC
  • Species
    Mouse, Mouses
  • Alternative name
    Rabbit anti Mouse IgG2b secondary antibody (FITC)
  • Alternative technique
    rabbit-anti, murine, antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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