Rabbit anti Mouse IgG2a biotin

  • Catalog number
    43R-IR031BT
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Rabbit anti-mouse IgG2a (biotin) was raised in rabbit using murine IgG2a heavy chain ..
  • Raised in
    Rabbit
  • Specificity
    Murine IgG2a
  • Cross Reactivity
    Should be determined by the end user
  • Conjugate Tag
    Biotin
  • Method of Purification
    Rabbit anti Mouse IgG2a (biotin) was purified by affinity chromatography.
  • Form Buffer
    Lyophilized in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and protease free) and 0.01% NaN3.
  • Storage
    Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; IHC; WB
  • Usage Recommendations
    ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Oryctolagus cuniculus
  • About
    Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Gene target
    IgG2a  
  • Short name
    Rabbit anti Mouse IgG2a
  • Technique
    anti mouse, Rabbit, Mouse, anti, antibody to, anti mouse antibodies, mouses
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG2a
  • Label
    Biotin
  • Species
    Mouse, Mouses
  • Alternative name
    Rabbit anti Mouse IgG2a secondary antibody (biotin)
  • Alternative technique
    rabbit-anti, murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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