Rabbit anti Mouse IgG H + L rhodamine

• Catalog number
  43R-1419
• Price
  Please ask
• Size
  1 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Purified Mouse IgG (H&L)
• Raised in
  Rabbit
• Specificity
  This antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin mouse serum proteins.
• Conjugate Tag
  Rhodamine
• Grade Purity
  Grater than 95% pure
• Method of Purification
  Affinity purified using solid phase Mouse IgG (H&L)
• Source
  Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml
• Storage
  Store at 2-8 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  FC; IF; IHC
• Usage Recommendations
  The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Oryctolagus cuniculus

• Gene target
  rhodamine  
• Short name
  Rabbit anti Mouse IgG H + L rhodamine
• Technique
  anti mouse, Rabbit, Mouse, anti, IgG, antibody to, anti mouse antibodies, IgGs, mouses
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Label
  Rhodamine
• Species
  Mouse, Mouses
• Alternative name
  Rabbit anti Mouse IgG (H + L) secondary antibody (rhodamine)
• Alternative technique
  rabbit-anti, murine, antibodies, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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