Rabbit anti phospho-VEGFR2 [pY1214]

  • Catalog number
    X2034P
  • Price
    Please ask
  • Size
    10MinibLots
  • Category
    Primary Antibodies
  • Long description
    Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200 kDa member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation. Phosphorylation of tyrosine 1214 is reported to play a critical role in regulation of receptor autophosphorylation and stimulation of intrinsic tyrosine kinase catalytic activity.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP2 protein and mouse myeloma Ag8563 cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR2 protein. The final product is generated by affinity chromatography using a VEGFR2-derived peptide that is phosphorylated at tyrosine 1214.
  • Clone
    Polyclonal
  • Antigen antibody binding interaction
    Rabbit anti phospho-VEGFR2 [pY1214] Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Human, Mouse
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot
  • Antibody s suited for
    Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed paraffin-embedded tissue sections (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Talvensaari-Mattila, A., et al. (2005). Prognostic value of matrix metalloproteinase-2 (MMP-2) expression in endometrial endometrioid adenocarcinoma. Anticancer Res. 25(6B);4101-4105._x000B__x000B_2. Honkavuori, M., et al. (2007). MMP-2 expression associates with CA125 and clinical course in endometrial carcinoma. Gynecol. Oncol. 104(1);217-221.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • About
    Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    KDR
  • Short name
    Rabbit anti phospho-VEGFR2 [pY1214]
  • Technique
    Rabbit, anti, antibody to
  • Host
    Rabbit, Rabbits
  • Isotype
    not specified
  • Label
    unlabeled
  • Alternative name
    production species: rabbit antibody to phosphorilated-VEGFR2 [pY1214]
  • Alternative technique
    rabbit-anti, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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