Rabbit anti phospho-VEGFR2 [pY1214]
-
Catalog number
X2034P
-
Price
Please ask
-
Size
10MinibLots
-
-
Category
Primary Antibodies
-
Long description
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200 kDa member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation. Phosphorylation of tyrosine 1214 is reported to play a critical role in regulation of receptor autophosphorylation and stimulation of intrinsic tyrosine kinase catalytic activity.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP2 protein and mouse myeloma Ag8563 cells.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR2 protein. The final product is generated by affinity chromatography using a VEGFR2-derived peptide that is phosphorylated at tyrosine 1214.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti phospho-VEGFR2 [pY1214] Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human, Mouse
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot
-
Antibody s suited for
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed paraffin-embedded tissue sections (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
-
Storage
-20ºC
-
Relevant references
1. Talvensaari-Mattila, A., et al. (2005). Prognostic value of matrix metalloproteinase-2 (MMP-2) expression in endometrial endometrioid adenocarcinoma. Anticancer Res. 25(6B);4101-4105._x000B__x000B_2. Honkavuori, M., et al. (2007). MMP-2 expression associates with CA125 and clinical course in endometrial carcinoma. Gynecol. Oncol. 104(1);217-221.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
KDR
-
Short name
Rabbit anti phospho-VEGFR2 [pY1214]
-
Technique
Rabbit, anti, antibody to
-
Host
Rabbit, Rabbits
-
Isotype
not specified
-
Label
unlabeled
-
Alternative name
production species: rabbit antibody to phosphorilated-VEGFR2 [pY1214]
-
Alternative technique
rabbit-anti, antibodies
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products