-
Category
Primary Antibodies
-
Long description
Vav proteins belong to the guanidine nucleotide exchange factor family of proteins. These proteins couple receptors to Rho-GTPases. To date, three members of the Vav family have been identified in mammalian cells: Vav1, Vav2, and Vav3. Vav proteins contain multiple protein binding motifs and act as adaptor proteins linking cell surface receptors to downstream signaling proteins. Vav3 transcripts are ubiquitously expressed in wide variety of cell lines including hematopoietic cells, suggesting its role in lymphocyte development and antigen receptor mediated activation of NFAT and NF B. Vav3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC , implicating a critical role in cytoskeletal rearrangement. Vav3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of Vav3.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP450 4F11 protein and mouse myeloma Ag8563 cells.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Vav3. The final product is generated by affinity chromatography using a Vav3-derived peptide that is phosphorylated at tyrosine 173.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti phospho Vav3 [pY173] Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human, Mouse
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot
-
Antibody s suited for
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
-
Storage
-80ºC
-
Relevant references
1. Kumarakasingham, M., et al. (2005). Cytochrome p450 profile of colorectal cancer: Identification of markers of prognosis. Clin. Cancer Res. 11(10);3758-3765._x000B__x000B_2. Cui, X.,et al. (2000). A novel human cytochrome P450 4F isoform (CYP4F11): cDNA cloning, expression and genomic structural characterization. Genomics. 68(2);161-166._x000B__x000B_3. Kalsotra, A., et al. (2004). Expression and characterization of human cytochrome P450 4F11: Putative role in the metabolism of therapeutic drugs and eicosanoids. Toxicol. Appl. Pharmacol. 199(3);295-304.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
