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Category
Primary Antibodies
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Long description
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signaling by growth factors, extracellular matrix and stress signals. Indeed, FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine phosphorylation of FAK is associated with positive regulation and growth promotion, while serine phosphorylation is associated with negative regulation, often during mitosis or cell adhesion. Phosphorylation of serine 722 may play a role in negatively regulating the binding of FAK to p130Cas.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP9 protein and mouse myeloma Ag8563 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR2 protein. The final product is generated by affinity chromatography using a VEGFR2-derived peptide that is phosphorylated at tyrosine 951.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti phospho Focal Adhesion Kinase (FAK) (pS722) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formalin-fixed paraffin-embedded tissues (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Wang, L. et al. (2006). Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J. Neurosci. 26(22);5996-6003_x000B__x000B_2. Solmiari, S.B., et al. (2006). Circulating MMP2 and MMP9 in breast cancer -- potential role in classification of patients into low risk, high risk, benign disease and breast cancer categories. Int. J. Cancer. 119(6);1403-1411_x000B__x000B_3. Chen, X., et al. (2005). Increased plasma MMP9 in integrin alpha1-null mice enhances lung metastasis of colon carcinoma cells. Int. J. Cancer. 116(1);52-61
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Rabbit anti phospho Focal Adhesion Kinase (FAK) (pS722). This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
