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Category
Primary Antibodies
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Long description
C-FOS [pT232] PAb; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat) c-Fos [pT232]. This antibody is validated for use in Western Blot. Anti-c-Fos [pT232] recognizes the expressed product of the FOS gene._x000B_Catalog number 44280G is same antibody as former catalog number 44280. 44280G contains 50% glycerol, which improves the stability and longevity of this antibody._x000B__x000B_c-Fos is a ~60 kDa immediate early gene product and a member of AP-1 family. c-Fos dimerizes with c-Jun to form the transcriptionally active complex AP-1, and functions in regulating cell proliferation and differentiation. c-Fos expression and activation are induced in many tumors (including breast cancer), and in response to a variety of stimuli (including growth factors, cytokines and stress). ERK1&2 mediate growth factor-induced c-Fos induction and transcripional activation by directly phosphorylating the carboxyl terminal domain of c-Fos. c-Fos is phosphorylated by Ras on threonine 232, the homologue of serine 73 of c-Jun, which results in augmenting its transcriptional activity.
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Antibody come from
Synthetic peptide derived from the rat sphingosine kinase 1, long form, protein.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Fos. The final product is generated by affinity chromatography using a c-Fos-derived peptide that is phosphorylated at threonine 232.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti phospho c-Fos [pT232] Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody suitable for Western blots (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Venkataraman, K., et al. 'Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient.' Biochem. J., 2006, 397, 461-471_x000B__x000B_2. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
