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Category
Primary Antibodies
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Long description
c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.
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Antibody come from
n/a
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Other description
Provided in 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 20 mM glutathione, and 10% glycerol. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti phospho c-c-Abl [pY412] Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, (Mouse)
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 10.2 pmol/min/µg. One U =1 pmol/min, Assay condition: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM HDAC substrate 1, 500 µM NAD+ and Sirtuin 1. Incubation condition: 30 min at 37¼C followed by treatment with 50 µl of HDAC developer for 15 min at RT.
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Storage
-20ºC
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Relevant references
1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877._x000B_ _x000B_2. Solomon, J.M. et al(2006) Mol. Cell. Biol. 26, 28-38._x000B__x000B_3. UCSC Genome Browser-more Specific for Sirtuin 1:_x000B_ details Gen db=hg18&hgg_gene=NM_012238&hgg_chrom=chr10&hgg_start=69314432&hgg_end=69348147, Accessed March 13, 2007.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
