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Category
Primary Antibodies
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Long description
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.
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Antibody come from
Peptide derived from the N-terminal of the alpha1-subunit of glycine receptor
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Other description
Lyophilized. Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti MEK1 (Thr292) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Dot Blot
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Antibody s suited for
Antibody provided lyophilized. Should be reconstitued in 100 µl of phosphate buffered saline. After reconstitution, antibody should be aliquoted and stored at -20¼C. Antibody can be used for Westen blot and immunohistochemistry (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1. Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964._x000B__x000B_2. Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798._x000B__x000B_3. Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220._x000B__x000B_4. Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, WŠssle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648._x000B__x000B_5. Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389._x000B__x000B_6. Schofield CM, Jenkins A, Harrison NL (2003) A highly conserved aspartic acid residue in the signature disulfide loop of the +1 subunit is a determinant of gating in the glycine receptor. J Biol Chem 278:34079-34083.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
