Rabbit anti Human IgM mu chain FITC

• Catalog number
  43R-1277
• Price
  Please ask
• Size
  1 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Purified Human IgM (mu chain)
• Raised in
  Rabbit
• Specificity
  This antibody reacts with heavy (mu) chains on human IgM.
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
• Conjugate Tag
  FITC
• Grade Purity
  Grater than 95% pure
• Method of Purification
  Affinity purified using solid phase Human IgG (H&L)
• Source
  Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
• Storage
  Store at 2-8 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  FC; IF; IHC
• Usage Recommendations
  The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  chain  
• Gene symbol
  JCHAIN, AP2M1
• Short name
  Rabbit anti IgM mu chain FITC
• Technique
  anti human, Rabbit, anti, FITC, IgM, antibody to, anti human antibodies, Fluorescein, IgMs
• Host
  Rabbit, Rabbits
• Isotype
  IgM, IgM
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  Rabbit anti Human IgM (mu chain) secondary antibody (FITC)
• Alternative technique
  rabbit-anti, antibodies, fluorescine, immunoglobulin-m

Gene info

• Identity
  HGNC:5713
• Gene
  JCHAIN
• Long gene name
  joining chain of multimeric IgA and IgM
• Synonyms gene
  • IGJ
• Synonyms gene name
  • immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
• Synonyms
  • IGCJ
  • JCH
• Synonyms name
  • immunoglobulin J chain
  • IgJ chain
  • J chain
• GenBank acession
  • M12759
• Locus
  4q13.3
• Discovery year
  2001-06-22
• Entrez gene record
  3512
• Pubmed identfication
  • 3016707
  • 2984306
• RefSeq identity
  • NM_144646
• VEGA ID
  OTTHUMG00000129909

Gene info

• Identity
  HGNC:564
• Gene
  AP2M1
• Long gene name
  adaptor related protein complex 2 subunit mu 1
• Synonyms gene
  • CLAPM1
• Synonyms gene name
  • adaptor related protein complex 2 mu 1 subunit
• Synonyms
  • AP50
  • mu2
• Synonyms name
  • clathrin-associated/assembly/adaptor protein, medium 1
  • plasma membrane adaptor AP-2 50kDA protein
  • clathrin coat adaptor protein AP50
  • clathrin adaptor complex AP2, mu subunit
  • HA2 50 kDA subunit
  • clathrin assembly protein complex 2 medium chain
  • AP-2 mu 2 chain
• GenBank acession
  • U36188
• Locus
  3q27.1
• Discovery year
  2000-09-01
• Entrez gene record
  1173
• Pubmed identfication
  • 8595912
• RefSeq identity
  • NM_004068
• Classification
  • Adaptor related protein complex 2
• VEGA ID
  OTTHUMG00000156822

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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