Rabbit anti Human IgM FITC

• Catalog number
  43C-CB9101
• Price
  Please ask
• Size
  1.5 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Rabbit anti-human IgM (FITC) was raised in rabbit using human IgM (Fc5mu) ..
• Raised in
  Rabbit
• Specificity
  Human IgM
• Cross Reactivity
  Should be determined by the end user
• Conjugate Tag
  FITC
• Method of Purification
  Rabbit anti Human IgM (FITC) was purified by affinity chromatography.
• Form Buffer
  Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
• Storage
  Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; FC; IF
• Usage Recommendations
  ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Short name
  Rabbit anti IgM FITC
• Technique
  anti human, Rabbit, anti, FITC, IgM, antibody to, anti human antibodies, Fluorescein, IgMs
• Host
  Rabbit, Rabbits
• Isotype
  IgM, IgM
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  Rabbit anti Human IgM secondary antibody (FITC)
• Alternative technique
  rabbit-anti, antibodies, fluorescine, immunoglobulin-m

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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