-
Category
Primary Antibodies
-
Long description
GFAP (55 kD) is selectively located in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the prolifeRation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’s disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.
-
Antibody come from
K39 is a Rabbit polyclonal antiSerum derived by immunization of a Rabbit with a glial fibrillary acidic protein preparation from Human spinal cord.
-
Other description
Each vial contains 100 ul Rabbit polyclonal Serum containing 0.09% sodium azide.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti Glial fibrillary acidic protein (K39) Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human,Mouse,Rat,Zebrafish
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
K39 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.
-
Research interest
Cancer,Cytoskeleton,Neurobiology,Stem cell research
-
Application
Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting, Immunocytochemistry
-
Antibody s suited for
K39 is suitable for immunoblotting, immunocytochemistry on permeabilized cells and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
-
Storage
Store at 4oC, or in small aliquots at –20oC.
-
Relevant references
Ramaekers, F. C., Puts, J. J., Moesker, O., Kant, A., Huysmans, A., Haag, D., Jap, P. H., Herman, C. J., and Vooijs, G. P. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview, Histochem J 15, 691-713. Herpers, M. J., Ramaekers, F. C., Aldeweireldt, J., Moesker, O., and Slooff, J. (1986). Co-expression of glial fibrillary acidic protein- and vimentin-type intermediate filaments in Human astrocytomas, Acta Neuropathol 70, 333-9.
-
Protein number
P14136
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus