Rabbit anti Escherichia coli Phosphatase alkaline

  • Catalog number
    NE124/7S
  • Product name
    Rabbit anti Escherichia coli Phosphatase alkaline
  • Size
    10 mg
  • Price
    224.31
  • Supplier
    nordc
  • Category
    Primary Antibodies
  • Long description
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
  • Antibody come from
    Alkaline phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  • Clone
    Polyclonal
  • Antigen-antibody binding interaction
    Rabbit anti Escherichia coli Phosphatase alkaline Antibody
  • Antibody is raised in
    Rabbit
  • Antibody's reacts with
    Bacteria
  • Antibody's reacts with these species
    Cross-reactivities anti enzymes of other sources may occur but have not been determined.
  • Antibody's specificity
    IgG fraction of polyclonal Rabbit antiSerum to alkaline phosphatase from Escherichia coli
  • Research interest
    Enzymes, Microbiology
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody's suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/80,000
  • Storage
    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
  • Relevant references
    no information yet
  • Protein number
    P00634
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • group_crc32
    493864418
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • About
    Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Rabbit anti Escherichia coli Phosphatase alkaline
  • Technique
    Rabbit, antibody to
  • Host
    Rabbit, Escherichia coli, Rabbits
  • Alternative name
    production species: rabbit antibody to Escherichia coli Phosphatase alkaline
  • Alternative technique
    rabbit-anti
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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