Rabbit Anti-Nitrotyrosine Polyclonal Antibody
Type of the product
Western blot, ELISA
100 μg of affinity purified antibody at 1 mg/mL in 75 mM PBS, pH 7.2, containing 0.5 mM EDTA and 0.02% NaN3
The product Rabbit Anti-Nitrotyrosine Polyclonal Antibody must be shipped on blue ice packages to retain its quality and activity during the transportation.
The product Rabbit Anti-Nitrotyrosine Polyclonal Antibody can be kept at minus 20 degrees Celsius for 12 months upon receipt.
This antibody is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Cellular biology labs they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Cellular biology labs. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cellular biology labs adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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