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Rabbit Anti-Human HSP40 Polyclonal: PE/ATTO 594

  • Catalog number
    SPC-100D-P594
  • Product name
    Rabbit Anti-Human HSP40 Polyclonal: PE/ATTO 594
  • Size
    100 µl
  • Price
    470.36
  • Supplier
    StressMark antibodies
  • Immunogen
    Recombinant purified human HSP40
  • Antibody's target
    Human HSP40
  • Antibody's full description
    Rabbit Anti-Human HSP40 Polyclonal
  • Primary research fields
    Cancer, Heat Shock
  • Antibody's category
    Polyclonal Antibodies
  • Antibody's other name
    DnaJ (HSP40) homolog subfamily B member 1 Antibody, DNAJ1 Antibody, DNAJB1 Antibody, HDJ1 Antibody, HSPF1 Antibody
  • Verified applications
    WB, ICC/IF, IP
  • Raised in
    Rabbit
  • Antibody's reactivity
    Human
  • Antibody's recommended dilutions for use
    WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody's purified from
    Rabbit Antiserum
  • Recommended buffer for storage
    Rabbit Antiserum
  • Antibody's concentration
    See included datasheet or contact our support service
  • Antibody's specificity
    Detects ~40kDa.
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.5 µg/ml of SPC-100 was sufficient for detection of HSP40 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
  • Bibliography
    1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86. 3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785. 5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781. 6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_006136.1
  • Gene number
    3337
  • Protein number
    P25685
  • PubMed number
    19240134
  • Tested applications
    Immunoprecipitation
  • Tested reactivity
    Human
  • Antibody's datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human HeLa cell lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-100). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-100) at 1:1000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-Hsp40 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-100) | Rabbit Anti-HSP40 Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-100) | Rabbit Anti-Hsp40 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-100)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight (kg)
    1.4
  • Net weight (g)
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Rabbit Anti- HSP40 Polyclonal: PE/ATTO 594
  • Technique
    anti-Human, antibody to, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Label
    PE
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit antibody to-H. sapiens HSP40 polyclonal: peroxidase/ATTO 594
  • Alternative technique
    polyclonals
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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