-
Immunogen
A synthesized peptide derived from human CD31
-
Antibody s target
Human CD31
-
Antibody s full description
Rabbit Anti-Human CD31 Polyclonal
-
Primary research fields
Cardiovascular System, Blood, Platelets, Cell Signaling, Cytoskeleton, Cell adhesion, Angiogenesis, Growth Factors, Angiogenic Factors, Stem Cells, Mesenchymal Stem Cells, MSC Surface Molecules, Hematopoietic Progenitors, Lymphoid, T Lymphocytic Lineage, Myeloid, Monocytic Lineage, Stem Cells, Neutrophil Lineage, Thrombocytic Lineage, Cardiovascular Markers, Endothelial Cells, Atherosclerosis, Vascular Inflammation, Leukocyte Recruitment, Cancer, Invasion/microenvironment, Immunology, Cell Type Markers, CD Markers
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PEC1 Antibody, PECA1 Antibody, PECAM Antibody, PECAM1 Antibody, Platelet endothelial cell adhesion molecule Antibody, GPIIA Antibody
-
Verified applications
WB, IHC, ICC/IF
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat
-
Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects endogenous levels of total CD31
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1283 was sufficient for detection of CD31 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Membrane, Cell Junction
-
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
-
Scientific context
CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma, and carcinomas. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.
-
Bibliography
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219. 4. Ganjei-Azar, Parvin (2007). Color Atlas of Immunocytochemistry in Diagnostic Cytology. [New York]: Springer Science+Business Media, LLC. p. 47.
-
Released date
6/Sep/2016
-
NCBI number
NP_000433.4
-
Gene number
5175
-
Protein number
P16284
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human Jurkat cell lysates showing detection of ~82kDa CD31 protein using Rabbit Anti-CD31 Polyclonal Antibody (SPC-1283). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-CD31 Polyclonal Antibody (SPC-1283) at 1:1000. Predicted/Observed Size: ~82kDa. Rabbit Anti-CD31 Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1283)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Anti-FITC Antibody
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
