Mouse IgG2a FITC

• Catalog number
  65C-CH1008
• Price
  Please ask
• Size
  100 µg

• Category
  Immunoglobulins
• Antibody Subtype
  Conjugated Immunoglobulins
• Area of research
  Immunology
• Reactivity
  Mouse
• Conjugate Tag
  FITC
• Method of Purification
  Mouse IgG2a (FITC) was purified by affinity chromatography.
• Form Buffer
  Lyophilized from 0.01M Na3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% thimerisol. I mMunoglobulin and protease free.
• Storage
  Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  IF
• Usage Recommendations
  Optimal conditions to be determined by end user
• URL
• More info
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  IgG2a  
• Short name
  Mouse IgG2a FITC
• Technique
  Mouse, FITC, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG2a
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  Purified Mouse IgG2a FITC conjugate
• Alternative technique
  murine, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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