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Category
Primary Antibodies
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Long description
This antibody recognizes a protein of 45 kDa, identified as MEK1 (also known as ERK kinase 1, MAPK kinase 1, and MKK1). MEK1 is a member of a family of tyrosine/threonine protein kinases that activate the ERK1&2/MAPK enzymes by phosphorylating both the threonine and tyrosine residues of the threonine Ð glutamic acid Ð tyrosine (TEY) motif located within the activation loop. MEK1 shares approximately 80% homology with the closely related dual specificity protein kinase MEK2. Active Raf family members phosphorylate MEK1 at serines 218 and 222, thereby activating it. Serine 298 of MEK1 is phosphorylated by PAK proteins, which promotes MEK1 binding to c-Raf and its subsequent phosphorylation of MEK1, leading to activation of the MAPK pathway. This antibody also recognizes MEK2, but not MEK3.
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Antibody come from
Recombinant human STAT1 expressed in E. coli.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1% BSA and 0.08% sodium azide. Protein A/G Chromatography
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Clone
263P15
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Antigen antibody binding interaction
Mouse anti MEK1 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, ELISA
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Antibody s suited for
Antibody can be used for Western blotting (0.5-1 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Burysek, L., T. Syrovets, and T. Simmet (2002) The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and Janus kinase (JAK)/STAT signaling pathways. J. Biol. Chem. 277(36):33509-33517 (cites the use of this antibody)._x000B__x000B_2. Schindler, C. and J.E. Darnell Jr. (1995) Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu. Rev. Biochem. 64:621-651._x000B__x000B_3. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334._x000B__x000B_4. Wen, Z. (1995) Maximal activation of transcription by Stat 1 and Stat 3 requires both tyrosine and serine phosphorylation. Cell 82(2):241-250.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
