Mouse anti Lamin B1

• Catalog number
  MUB1103S
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Lamins play a crucial role in the maintenence of nuclear structure, gene regulation and signalling from cytoplasm to nucleus. Mutations in lamins have been associated with a number of pathologies; the so-called laminopathies.
• Antibody come from
  119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.
• Other description
  Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
• Clone
  119D5-F1
• Antigen antibody binding interaction
  Mouse anti Lamin B1 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Bovine,Canine,Human,Mouse,Rabbit,Rat,Sheep,Zebrafish
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  119D5-F1 reacts with an epitope loCated C-terminal of residue 231 in lamin B1.
• Research interest
  Cytoskeleton, Neurobiology
• Application
  Flow Cytometry,Immunocytochemistry,Western blotting, Immunohistochemistry (Frozen), ELISA
• Antibody s suited for
  119D5-F1 is suitable for immunocytochemistry on permeabilised cells, immunohistochemistry on frozen tissues, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Broers, J.L., Ramaekers, F.C.S., Bonne, G., Hutchison, C.J. (2006). Nuclear Lamins: laminopathies and their role in premature aging. Physiol Rev 86, 967-1008. Weaver, V. M., Carson, C. E., Walker, P. R., Chaly, N., Lach, B., Raymond, Y., Brown, D. L., and Sikorska, M. (1996). Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes, J Cell Sci 109, 45-56. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95. Broers, J. L., Bronnenberg, N. M., Kuijpers, H. J., Schutte, B., Hutchison, C. J., and Ramaekers, F. C. (2002). Partial cleavage of A-type lamins concurs with their total disintegRation from the nuclear lamina during apoptosis. Eur J Cell Biol 81, 677-691.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Lamin  
• Gene symbol
  LMNB1
• Short name
  Mouse anti Lamin B1
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Lamin B1
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6637
• Gene
  LMNB1
• Long gene name
  lamin B1
• GenBank acession
  • L37737
• Locus
  5q23.2
• Discovery year
  1995-03-28
• Entrez gene record
  4001
• Pubmed identfication
  • 7557986
  • 8838815
• RefSeq identity
  • NM_005573
• Classification
  • Lamins
• VEGA ID
  OTTHUMG00000128969

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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