Mouse anti Lamin A

• Catalog number
  MUB1101P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
• Antibody come from
  133A2 is a Mouse monoclonal IgG3/κ antibody obtained from fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with partially purified recombinant Human lamin A.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. Since this IgG3 antibody has to be purified from the culture medium by ion-exchange chromatography it will contain remnants of bovine serum albumin.
• Clone
  133A2
• Antigen antibody binding interaction
  Mouse anti Lamin A Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Bovine,Canine,Human,Mouse,Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  133A2 recognizes an epitope loCated between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A.
• Research interest
  Cytoskeleton, Neurobiology
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
• Antibody s suited for
  133A2 is suitable for immunoblotting, immunocytochemistry on permeabilized cells, immunohistochemistry on frozen sections and paraffin embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunocytochemistry, and immunohistochemistry with fluorescent secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Hozak, P., Sasseville, A. M., Raymond, Y., and Cook, P. R. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in Human cells, J Cell Sci 108, 635-44. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Neri, L. M., Raymond, Y., Giordano, A., Borgatti, P., Marchisio, M., Capitani, S., and Martelli, A. M. (1999). Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions, J Cell Biochem 75, 36-45. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95. Broers, J. L., Machiels, B. M., van Eys, G. J., Kuijpers, H. J., Manders, E. M., van Driel, R., and Ramaekers, F. C. (1999). Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins, J Cell Sci 112, 3463-75. Broers, J. L., Bronnenberg, N. M., Kuijpers, H. J., Schutte, B., Hutchison, C. J., and Ramaekers, F. C. (2002). Partial cleavage of A-type lamins concurs with their total disintegRation from the nuclear lamina during apoptosis. Eur J Cell Biol 81, 677-691. De Sandre-Giovannoli, A., Bernard, R., Cau, P., Navarro, C., Amiel, J., Boccaccio, I., Lyonnet, S., Stewart, C. L., Munnich, A., Le Merrer, M., and Levy, N. (2003). Lamin a trunCation in Hutchinson-Gilford progeria. Science 300, 2055. Eriksson, M., Brown, W. T., Gordon, L. B., Glynn, M. W., Singer, J., Scott, L., Erdos, M. R., Robbins, C. M., Moses, T. Y., Berglund, P., et al. (2003). Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome. Nature 423, 293-298.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Lamin  
• Gene symbol
  LMNA, MLIP
• Short name
  Mouse anti Lamin A
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG3
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Lamin A
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6636
• Gene
  LMNA
• Long gene name
  lamin A/C
• Synonyms gene
  • LMN1
  • CMD1A
  • LGMD1B
  • PRO1
  • LMNL1
• Synonyms gene name
  • cardiomyopathy, dilated 1A (autosomal dominant)
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • progeria 1 (Hutchinson-Gilford type)
  • lamin A/C-like 1
• Synonyms
  • HGPS
  • MADA
• Synonyms name
  • mandibuloacral dysplasia type A
• GenBank acession
  • BC014507
• Locus
  1q22
• Discovery year
  1992-04-09
• Entrez gene record
  4000
• Pubmed identfication
  • 8511676
  • 8838815
  • 12702809
• RefSeq identity
  • NM_170707
• Classification
  • Lamins
• VEGA ID
  OTTHUMG00000013961
• Locus Specific Databases
  • Inherited Peripheral Neuropathies Mutation Database
  • Leiden Muscular Dystrophy Pages
  • UMD Locus Specific Databases
  • Leiden Muscular Dystrophy pages
  • LRG_254

Gene info

• Identity
  HGNC:21355
• Gene
  MLIP
• Long gene name
  muscular LMNA interacting protein
• Synonyms gene
  • C6orf142
• Synonyms gene name
  • chromosome 6 open reading frame 142
• Synonyms
  • MGC18257
  • CIP
• Synonyms name
  • muscle-enriched A-type lamin interacting protein
  • cardiac ISL1-interacting protein
• GenBank acession
  • AK055530
• Locus
  6p12.1
• Discovery year
  2003-06-09
• Entrez gene record
  90523
• Pubmed identfication
  • 21498514
• RefSeq identity
  • NM_138569
• VEGA ID
  OTTHUMG00000014891

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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