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Category
Primary Antibodies
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Long description
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein, a cytoskeletal protein that is located within the cytoplasm of astrocyctes and other glial cells e.g. Schwann cells and ependymal cells. In the central nervous system, GFAP is the major intermediate filament protein of mature astrocytes. GFAP plays an important role in maintaining the structure and stability of astrocytes during development and has also been shown to be involved in cellular communication, motility and mitosis. Mutations in GFAP give rise to Alexander disease, a rare and fatal disease of the central nervous system. Antibodies recognizing GFAP are commonly used to identify cells of astrocytic origin under normal and pathological conditions.
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Antibody come from
G-A-5 is a mouse monoclonal IgG1 antibody derived by fusion of PA1 mouse myeloma cells with spleen cells from BALB/c mice immunized with purified GFAP from porcine spinal cord.
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Other description
Each vial contains 100µl 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide.
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Clone
G-A-5
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Antigen antibody binding interaction
Mouse anti Glial fibrillary acidic protein (GFAP) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat, Pig, Chicken
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The antibody G-A-5 recognises GFAP in human, mouse, rat, porcine and chicken. The antibody reacts with the carboxyterminal cys II fragment of GFAP as determined by western blotting and does not cross react with vimentin
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Research interest
Cytoskeletal, neuroscience, cell cycle
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Application
Immunocytochemistry, Immunohistochemistry (Frozen, Paraffin), Western Blotting
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Antibody s suited for
The G-A-5 antibody is suitable for the detection of purified GFAP by Western blotting and detects a band of 51kDa in glioma cell lines. The antibody is also suitable for the detection of GFAP by Immunocytochemistry on permeabilized cells, and Immunohistology of frozen and formalin fixed paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
1. Debus E, Weber K, Osborn M. Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. Differentiation. (1983) 25:193 – 203.
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Protein number
P14136
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
