Mouse anti GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
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Catalog numberX2677M
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PricePlease ask
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionGAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate
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Antibody come fromSynthetic peptide derived from the human SUMO-1 protein.
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Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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CloneGA1R
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Antigen antibody binding interactionMouse anti GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) Antibody
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Antibody is raised inMouse
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Antibody s reacts withsee techfile
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWB, Immunostaining, Dot Blot, ELISA
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Antibody s suited forAntibody can be used for Western blotting (1:400 dilution) and immunohistochemistry (10-15µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Saitoh, H., et al. 'SUMO-1: wrestling with a new ubiquitin-related modifier.' Trends Biochem Sci 1997; 22(10):374-376_x000B__x000B_2. Muller, S., et al. 'Conjugation with the ubiquitin-related modifier SUMO-1 regulated the partitioning of PML within the nucleus.' EMBO J 1998; 17(1):61-70_x000B__x000B_3. Navascues J., et al. 'SUMO-1 transiently localizes to Cajal bodies in mammalian neurons.' J Struct Biol 2008; 163(2):137-146.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolGAPDH-DT, GAPDH, GAPDHS, GAPDHP15, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
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Short nameMouse anti GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameGAPDH divergent transcript
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Locus
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Discovery year2021-03-12
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Entrez gene record
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase
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Synonyms gene
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase, spermatogenic
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase pseudogene 15
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Synonyms gene
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Synonyms
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Locus
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Discovery year2003-11-26
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 14
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 12
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
