Mouse anti CD28

  • Catalog number
    0281
  • Price
    Please ask
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60%-80% T lymphocytes (95% of CD4 and 50% of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.
  • Antibody come from
    CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    B-23
  • Antigen antibody binding interaction
    Mouse anti CD28 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Research interest
    CD Marker
  • Application
    Flow Cytometry
  • Antibody s suited for
    PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Storage
    -20ºC
  • Relevant references
    1. CD28 is an inducible T cell surface antigen that transduces a proliferative signal in CD3+ mature thymocytes. Turka LA; Ledbetter JA; Lee K; June CH; Thompson CB J Immunol 1990 Mar ;144(5):1646-53 _x000B__x000B_2. CD28 ligation in T-cell activation: evidence for two signal transduction pathways. Ledbetter JA; Imboden JB; Schieven GL; Grosmaire LS; Rabinovitch PS; Lindsten T; Thompson CB; June CH Blood 1990 Apr ;75(7):1531-9 _x000B__x000B_3. T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1. Linsley PS; Clark EA; Ledbetter JA Proc Natl Acad Sci U S A 1990 Ju;87(13):5031-5 _x000B__x000B_4. Role of the CD28 receptor in T-cell activation. June CH; Ledbetter JA; Linsley PS; Thompson CB Immunol Today 1990 Ju;11(6):211-6 _x000B__x000B_5. Differences in surface phenotype and mechanism of action between alloantigen-specific CD8+ cytotoxic and suppressor T cell clones. Koide J; Engleman EG J Immunol 1990 Jan ;144(1):32-40 _x000B__x000B_6. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Linsley PS; Brady W; Grosmaire L; Aruffo A; Damle NK; Ledbetter JA J Exp Med 1991 Mar ;173(3):721-30 _x000B__x000B_7. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. Koulova L; Clark EA; Shu G; Dupont B J Exp Med 1991 Mar ;173(3):759-62
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    CD28  
  • Gene symbol
    CD28
  • Short name
    Mouse anti CD28
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to CD28 molecule
  • Alternative technique
    murine, antibodies
  • Alternative to gene target
    CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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