Mouse anti CD18

• Catalog number
  0181
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  LFA-1 beta is a leukocyte adhesion receptor to (ICAM's) intercellular adhesion molecules. LFA-1 beta is on virtually all peripheral blood lymphocytes, 95,000 M.W. surface antigen.
• Antibody come from
  n/a
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  3EB
• Antigen antibody binding interaction
  Mouse anti CD18 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  CD Marker
• Application
  Flow Cytometry
• Antibody s suited for
  PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  -20ºC
• Relevant references
  1. CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the alpha chain of fibrinogen. Loike, J.D., Sodeik, B., Cao, L., Leucona, S., Weitz, J.I., Detmers, P.A., Wright, S.D., Silverstein, S.C.. Proc. Natl. Acad. Sci. USA, 1991 Feb; 88(3):1044-8. _x000B__x000B_2. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Dustin, M.L. and Springer, T.A.. Nature. 1989:341:619. _x000B__x000B_3. Coupling of the adhesive receptor CD11/CD18 to functional enhancement of effector macrophage tissue factor response. Fan, S.T. and Edgington, T.S.. J. Clin. Invest. 1991 Ja:87(1):50-7. _x000B__x000B_4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med.1987;38:175. _x000B__x000B_5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15. _x000B__x000B_6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin -triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA, 1990 De;87(23):9472-6.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD18  
• Gene symbol
  ITGB2
• Short name
  Mouse anti CD18
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD18
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6155
• Gene
  ITGB2
• Long gene name
  integrin subunit beta 2
• Synonyms gene
  • CD18
  • MFI7
• Synonyms gene name
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Synonyms
  • LFA-1
  • MAC-1
• Synonyms name
  • complement component 3 receptor 3 and 4 subunit
• GenBank acession
  • AK222505
• Locus
  21q22.3
• Discovery year
  1986-01-01
• Entrez gene record
  3689
• RefSeq identity
  • NM_000211
• Classification
  • Integrin beta subunits
  • Complement system regulators and receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000090257
• Locus Specific Databases
  • ITGB2base: Mutation registry for Leukocyte adhesion deficiency I (LAD-I)
  • LRG_76

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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