Mouse anti alpha T-Catenin

  • Catalog number
    X1726M
  • Price
    Please ask
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    Catenins are proteins that connect cadherin/ -catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, T-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human Tcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking -catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells._x000B_The encoded protein has about the same predicted size (100 kDa) as other -catenins to which it shows an overall amino acid identity of 57%. In cells transfected with T-catenin cDNA, interaction with -catenin was demonstrated by coimmunoprecipitation._x000B_This antibody is specific for T-catenin and does not cross react with E-catenin or N-catenin by Western blot.
  • Antibody come from
    Human Flt3 (GenBank Accession No. NM_004119), (a.a. 564-end) with Nterminal His tag, MW=54.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
  • Other description
    25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
  • Clone
    892_24D2S
  • Antigen antibody binding interaction
    Mouse anti alpha T-Catenin Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot
  • Antibody s suited for
    Specific Activity: 225 pmol/min/µg. Assay condition: The enzyme reactionwas carried out for 1h at room temperature in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ35 and 200 µM of ATP. Substrate: 2 µM Tyr peptide 2 from Invitrogen. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
  • Storage
    -20ºC
  • Relevant references
    1. Takahashi,S., et al., Leuk. Res. 29 (8), 893899 (2005) _x000B__x000B_2. Levis,M., et al., Blood 106 (2), 673-680 (2005).
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    The Mouse anti alpha T-Catenin is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    CTNNA3
  • Short name
    Mouse anti alpha T-Catenin
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to a T-Catenin
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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