Goat anti Mouse IgG2b FITC

  • Catalog number
    43R-IG066ft
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Raised in
    Goat
  • Specificity
    Murine
  • Cross Reactivity
    Should be determined by the end user
  • Conjugate Tag
    FITC
  • Method of Purification
    Goat anti Mouse IgG2b (FITC)
  • Concentration
    1.0 mg/ml
  • Form Buffer
    PBS pH 7.2, with 0.2% BSA and 0.1% NaN3.
  • Storage
    Store at 4 deg C. DO NOT FREEZE.
  • Shipping conditions
    Blue Ice
  • Tested for
    ICC; IHC
  • Usage Recommendations
    ICC: 1:200-1:2,000, IHC: 1:200-1:2,000
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus, Mus musculus
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Gene target
    IgG2b  
  • Short name
    Goat anti Mouse IgG2b FITC
  • Technique
    anti mouse, Mouse, anti, FITC, Goat, antibody to, anti mouse antibodies, Fluorescein, goats, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgG2b
  • Label
    FITC
  • Species
    Mouse, Goats, Mouses
  • Alternative name
    Goat anti Mouse IgG2b secondary antibody (FITC)
  • Alternative technique
    murine, antibodies, caprine, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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