Rat anti Mouse IgG2b biotin
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Catalog number
43R-I118aBT
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rat anti-mouse IgG2b (biotin) was raised in rat using murine IgG ..
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Raised in
Rat
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Specificity
Murine
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Cross Reactivity
Should be determined by the end user
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Isotypes
IgG1 kappa
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Clone
LO-MG2b-2
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Conjugate Tag
Biotin
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Concentration
1.0 mg/ml
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Form Buffer
PBS with 0.1% NaN3 and 50% glycerol.
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Storage
Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
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Shipping conditions
Blue Ice
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Tested for
ELISA; IC
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Usage Recommendations
Optimal conditions to be determined by end user
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
anti Mouse IgG2b
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Technique
anti mouse, Mouse, anti, antibody to, anti mouse antibodies, mouses
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Host
Rat
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Isotype
IgG2b
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Label
Biotin
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Species
Mouse, Mouses, Rats
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Alternative name
Rat anti Mouse IgG2b secondary antibody (biotin)
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Alternative technique
murine, antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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