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SOD Activity Kit

  • Catalog number
    SKT-214-192
  • Product name
    SOD Activity Kit
  • Size
    2 x 96 well
  • Price
    600.88 USD
  • Supplier
  • Stock availability
    In Stock
  • Kit's description
    Quantitative colorimetric measurement of superoxide dismutase activity
  • Protein target
    SOD
  • Scientific context
    Short-lived and highly reactive oxygen species (ROS) such as O2·- (superoxide), ·OH (hydroxyl radical), and H2O2 (hydrogen peroxide) are continuously generated in vivo. In the resting state, the balance between antioxidants and oxidants is sufficient to prevent the disruption of normal physiologic functions; however, either increases in oxidants or decreases in antioxidants can disrupt this balance giving rise to elevated levels of reactive oxygen species (ROS) (1,2).The cellular levels of ROS are controlled by antioxidant enzymes and small molecule antioxidants. The major antioxidant enzymes, superoxide dismutases (SODs), including copper-zinc superoxide dismutase (Cu/ZnSOD, SOD1), manganese superoxide dismutase (MnSOD, SOD2) and extracellular superoxide dismutase (EC-SOD, SOD3), all play critical roles in scavenging O2·-. Decreased SOD activity results in elevated level of superoxide which in turn leads to decreased NO but increased peroxynitrite concentrations. The major intracellular SOD is a 32-kD copper and zinc containing homodimer (Cu/Zn SOD). The mitochondrial SOD (MnSOD) is a manganese-containing 93-kD homotetramer that is synthesized in the cytoplasm and translocated to the inner matrix of mitochondria. EC-SOD is the primary extracellular SOD enzyme and is highly expressed in many organs. Increased SOD activity levels are seen in Downs Syndrome (3) while decreased activity is seen in diabetes, Alzheimer’s disease, rheumatoid arthritis, Parkinson’s disease, uremic anemia, atherosclerosis, some cancers, and thyroid dysfunction (3-8).
  • Primary research area
    Cancer, Oxidative Stress, Cardiovascular System, Atherosclerosis, Neuroscience, Neurodegeneration, Alzheimer's Disease, Parkinson's Disease
  • Category
    Assay Activity Kits
  • Other name
    Cu/ZnSOD Activity Kit, SOD1 Activity Kit, MnSOD Activity Kit, SOD2 Activity Kit, EC-SOD Activity Kit, SOD3 Activity Kit
  • Brand name
    StressXpress®
  • Detection system
    Colorimetric Assay
  • Assay format
    Direct Enzyme Activity Assay
  • Main intended usage
    Colorimetric assay used to quantitatively measure SOD activity in a variety of samples.
  • Brief protocol
    The Superoxide Dismutase (SOD) Activity Kit is designed to quantitatively measure SOD activity in a variety of samples. The assay measures all types of SOD activity, including Cu/Zn, Mn, and FeSOD types. A bovine erythrocyte SOD standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in our specially colored Sample Diluent and added to the wells. The Substrate is added followed by Xanthine Oxidase Reagent and incubated at room temperature for 20 minutes. The Xanthine Oxidase generates superoxide in the presence of oxygen, which converts a colorless substrate in the Detection Reagent into a yellow colored product. The colored product is read at 450 nm. Increasing levels of SOD in the samples causes a decrease in superoxide concentration and a reduction in yellow product. The activity of the SOD in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of SOD activity per mL.
  • Kit contents
    Clear 96 well Half Area Plates, Superoxide Dismutase Standard, Assay Buffer, Xanthine Oxidase Buffer, Xanthine Oxidase Concentrate, Substrate Diluent, Substrate Concentrate
  • Assay precision
    Intra Assay Precision: Three samples diluted in Assay Buffer were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were: Sample 1- 0.407 U/mL, 4.6% CV Sample 2- 0.726 U/mL, 7.3% CV Sample 3- 1.203 U/mL, 16.8% CV Inter Assay Precision: Three samples diluted in Assay Buffer were run in duplicates in sixteen assays run over multiple days by four operators. The mean and precision of the calculated concentrations were: Sample 1- 0.356 U/mL, 10.5% CV Sample 2- 0.653 U/mL, 6.1% CV Sample 3- 1.277 U/mL, 13.8% CV
  • Assay cross-reactivity
    Species Independent
  • Samples to be used with this kit
    Serum, Heparin Plasma, EDTA Plasma, Cell Lysates, Tissue, Erythrocytes
  • Maximum samples to be used with this kit
    89 samples in duplicate
  • Assay duration
    See product datasheet or feel free to contact us
  • Kit's sensitivity
    0,044 U/ml
  • Assay detection limit
    0,0625 - 4 U/ml
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice
  • Bibliography
    1. Liocher, SI and Fridovich, I. Free Rad. Biol. Med., 2007, 42:1465-1469. 2. Imlay, JA. Ann Rev. Bichem., 2008, 77:755-776. 3. Torsdottir, G. et al. J.Neurol.Sci. 2010. 299(1-2):51-54. 4. Giacco, F & Brownlee, M. Circ.Res. 2010. 107(9):1058-1070. 5. Bae, S-C, et al. J.Amer.Coll.Nutr. 2003. 22(4):311-315. 6. Akbostanci, MC, et al. Acta Neurol.Belg. 2001. 101:180-183. 7. Shainkin-Kestenbaum, R, et al. Nephron. 1990. 55(3):251-253. 8. Saito, T. Hokkaido Igaku Zasshi. 1987. 62(2):257-268.
  • Release date
    17-Sep-2014
  • PubMed number
    Refer to PubMed
  • Tested applications
    No data available / currently testing
  • Tested reactivity
    No data available / currently testing
  • Resources available upon request
    Kit Booklet, MSDS
  • Product image link
  • Product image legend
    Typical Standard Curve for SOD Activity Kit (Enzyme Activity Assay) StressXpress® - SKT-214. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.0625 - 4 U/ml. | Linearity was determined by taking two samples, one with a high known SOD activity and the other with a lower SOD activity and mixing them in given ratios. The measured activities were compared to the expected values based on the ratios used. | Short-lived and highly reactive oxygen species (ROS) such as O2·-(superoxide), ·OH (hydroxyl radical), and H2O2 (hydrogen peroxide) are continuously generated in vivo. The cellular levels of ROS are controlled by antioxidant enzymes and small molecule antioxidants. The major antioxidant enzymes, superoxide dismutases (SODs), including copper-zinc superoxide dismutase (Cu/ZnSOD, SOD1), manganese superoxide dismutase (MnSOD, SOD2) and extracellular superoxide dismutase (EC-SOD, SOD3), all play critical roles in scavenging O2·-. Graph of the Typical Standard Curve for the SOD Activity Kit StressXpress - SKT-214 | Graph of the Linearity Recovery for the SOD Activity Kit StressXpress - SKT-214 | Diagram of the Reactive Oxygen Species (ROS) Formation for the SOD Activity Kit StressXpress - SKT-214
  • Warnings
    Non-hazardous
  • Country of production
    Canada
  • Total weight (kg)
    2
  • Net weight (g)
    300
  • Gene target
  • Short name
    SOD Activity Kit
  • Alternative name
    antioxidant enzyme Activity reagent
  • Alternative technique
    kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note:A blood protein (NSILA) which mimics the biological activity of insulin in serum, but is not suppressed by insulin antibodies. During acid-ethanol extraction of Cohn fraction III, 10% of the activity is found in the supernatant (NSILA-S) and the remaining activity in the precipitate (NSILA-P). The latter is a large molecular compound, much less stable than the soluble fraction. NSILA-S is a more potent growth factor than insulin and exhibits sulfation activity.
  • Tree numbers
    • D12.776.124.862.575
  • Qualifiers
    immunology, pharmacokinetics, biosynthesis, physiology, therapeutic use, secretion, toxicity, poisoning, administration & dosage, adverse effects, agonists, cerebrospinal fluid, chemical synthesis, contraindications, deficiency, chemistry, drug effects, urine, radiation effects, history, analysis, antagonists & inhibitors, classification, economics, genetics, isolation & purification, metabolism, pharmacology, standards, supply & distribution, ultrastructure
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