Human Oncostatin M (OSM) CLIA Kit, 96 well plate
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Catalog numberEKU08831
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PricePlease ask
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Size10x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits31.25-2000 pg/mL
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Sensitivity limit0,93 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP13725
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Alternate protein numberPlease refer to SwissProt
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Gene number5008
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Gene nameOncostatin M
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Alternate gene nameOS-M
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Oncostatin M (OSM). No significant cross-reactivity or interference between Oncostatin M (OSM) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Oncostatin M (OSM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Oncostatin M (OSM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Oncostatin M (OSM) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oncostatin M (OSM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oncostatin M (OSM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaCytokine;Tumor immunity;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Gene symbolOSM, CCM2, IFT172, KIF17
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Short nameOncostatin M (OSM) CLIA Kit, 96
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SpeciesHuman, Humans
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Alternative nameH. sapiens Oncostatin M (oncostatin M) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetoncostatin M, OSM and IDBG-4002 and ENSG00000099985 and 5008, growth factor activity, Extracellular, Osm and IDBG-142284 and ENSMUSG00000058755 and 18413, BT.45038 and IDBG-636338 and ENSBTAG00000016163 and 319086
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Gene info
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Identity
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Gene
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Long gene nameoncostatin M
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCCM2 scaffold protein
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Synonyms gene
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Synonyms gene name
- chromosome 7 open reading frame 22
- cerebral cavernous malformation 2
- CCM2 scaffolding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CCM adhesion complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameintraflagellar transport 172
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Synonyms gene name
- intraflagellar transport 172 homolog (Chlamydomonas)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-11-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- IFT-B2 complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekinesin family member 17
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kinesins
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VEGA ID