EZLabel™ Protein Biotin Labeling Kit
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Catalog number
K835-5
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Price
Please ask
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Size
For 5 x 1 mg of protein
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Description
Easy to use Kit to label upto 5 mg of Protein with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
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Summary
Easy to use Kit to label upto 5 mg of Protein with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
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Detection Method
280 nm (A280).
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Species Reactivity
N/A
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Applications
Labeled proteins can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis
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Sample Type
Proteins
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Features Benefits
All necessary reagents included. Fast and efficient labeling of proteins with Biotin
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Storage Conditions
+4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
BioVision’s EZLabel™ Protein-Biotin Labeling Kit provides an easy way to label proteins with Biotin in a user-friendly spin column format. Due to its high affinity for tetrameric avidin proteins, biotin is ideal for labeling proteins. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. Biotin-labeled protein can be directly used for multiple downstream applications including ELISA, western blot, Immunohistochemistry, Immunoassays, etc.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene target
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Short name
EZLabel™ Protein Labeling Kit
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Label
Biotin
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Alternative name
EZLabel™ Protein biotinilated Labeling reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Use of written, printed, or graphic materials upon or accompanying a drug container or wrapper. It includes contents, indications, effects, dosages, routes, methods, frequency and duration of administration, warnings, hazards, contraindications, side effects, precautions, and other relevant information.
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Tree numbers
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Qualifiers
trends, ethics, history, classification, economics, instrumentation, methods, standards, legislation & jurisprudence, statistics & numerical data
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