Human Angiotensin 1-7 (Ang1-7) CLIA Kit, 96 well plate
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Catalog numberEKU10051
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PricePlease ask
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Size96T
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Verified reactivityHomo sapiens (Human)
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Detection limits1.56-400 pg/mL
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Sensitivity limit0,64 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Assay duration2 hours
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Verified applicationsCLIA
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Assay classCompetitive
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ELISA detectionChemiluminescent
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Protein numberPlease refer to Uniprot
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Alternate protein numberPlease refer to SwissProt
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Gene numberPlease refer to GenBank
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Gene nameAngiotensin 1-7
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Alternate gene namePlease refer to NCBI
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Angiotensin 1-7 (Ang1-7). No significant cross-reactivity or interference between Angiotensin 1-7 (Ang1-7) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Angiotensin 1-7 (Ang1-7). A competitive inhibition reaction is launched between biotin labeled Angiotensin 1-7 (Ang1-7) and unlabeled Angiotensin 1-7 (Ang1-7) (Standards or samples) with the pre-coated antibody specific to Angiotensin 1-7 (Ang1-7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin 1-7 (Ang1-7) level in the sample or standard.
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaBiology
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Gene symbolMIR7-1, TRE-CTC1-7, TRV-CAC1-7, TRL-CAG1-7, PIRC34, PIRC33, PIRC32, TRK-TTT7-1, TRR-CCT7-1, TRI-AAT7-1
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Short nameAngiotensin 1-7 (Ang1-7) CLIA Kit, 96
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SpeciesHuman, Humans
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Alternative nameH. sapiens Angiotensin 1-7 (Ang1-7) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene nametRNA-Glu (anticodon CTC) 1-7
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Synonyms gene
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Synonyms gene name
- transfer RNA glutamic acid 19 (anticodon CUC)
- transfer RNA-Glu (CTC) 1-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon CAC) 1-7
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Synonyms
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Locus
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Discovery year2020-02-13
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon CAG) 1-7
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 26 (anticodon CAG)
- transfer RNA-Leu (CAG) 1-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 34
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 33
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 32
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nametRNA-Lys (anticodon TTT) 7-1
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Synonyms gene
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Synonyms gene name
- transfer RNA lysine 29 (anticodon UUU)
- transfer RNA-Lys (TTT) 7-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Arg (anticodon CCT) 7-1
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Synonyms gene
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Synonyms gene name
- transfer RNA arginine 30 (anticodon CCU) pseudogene
- transfer RNA-Arg (CCT) 7-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon AAT) 7-1
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 8 (anticodon AAU)
- transfer RNA-Ile (AAT) 7-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
